New Simple Sequence Length Polymorphism Markers
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New SSLP Primers

Each primer pair amplifies a unique segment of genomic DNA containing a simple tandem repeat. The PCR products differ in length between Columbia and Landsberg erecta, and often, other ecotypes. All of these primers are available at low cost from Research Genetics Inc., 2130 Memorial Parkway, Huntsville, Alabama 35801. Telephone 800-533-4363. FAX 205-536-9016. We have no connection with Research Genetics Inc., other than as a customer. Unrestricted use of these sequences and free transfer of the information between laboratories is encouraged.

  • SSLP marker map

  • SSLP Ecotype Summary

    chromosome 1 (between m241 and g3786)


    Ath SRP54A-L: TGAATTATGGAATCAATGTTCG
    Ath SRP54A-R: AAAAGGAACCCTACCAAAAACA


    chromosome 1 (between m235 and g17286)


    Ath ZFPG-L: TTGCGTTTCCACATTTGTTT
    Ath ZFPG-R: TGGGTCAATTCACATGTAGAGA

    chromosome 1 (between m201 and m215)


    Ath S0392-R TTTGGAGTTAGACACGGATCTG
    Ath S0392-L GTTGATCGCAGCTTGATAAGC


    chromosome 1 (between m201 and nga248)


    nga392L TTGAATAATTTGTAGCCATG
    nga392R GGTGTTAAATGCGGTGTTC


    chromosome 1 (between m253 and GAPB)


    T27K12-Sp6F (BAC end): GGACAACGTCTCAAACGGTT
    T27K12-Sp6R (BAC end): GGAGGCTATACGAATCTTGACA


    chromosome 1 (between AthATPASE and g17311)


    nga692L TTTAGAGAGAGAGAGCGCGG
    nga692R AGCGTTTAGCTCAACCCTAGG


    chromosome 2 (between NOR2 and m246)


    pnga 1145-L: CCTTCACATCCAAAACCCAC
    pnga 1145-R: GCACATACCCACAACCAGAA



    chromosome 2 (between g6842 and nga1126)


    Ath GPa1-L: ATCTCTTATCGTCACGAGAGGC
    Ath GPa1-R: TAAGCAAAGGGGAAGACGAA

    chromosome 2 (between GPa1 and er)


    nga1126L CGCTACGCTTTTCGGTAAAG
    nga1126R GCACAGTCCAAGTCACAACC


    chromosome 2 (between m323 and m283)


    nga361L AAAGAGATGAGAATTTGGAC
    nga361R ACATATCAATATATTAAAGTAGC


    chromosome 2 (between g4514 and m336)


    AthBIO2L TGACCTCCTCTTCCATGGAG
    AthBIO2R TTAACAGAAACCCAAAGCTTTC


    chromosome 2 (between m336 and yUP21A12L)


    Ath UBIQUE-L: AGGCAAATGTCCATTTCATTG
    Ath UBIQUE-R: ACGACATGGCAGATTTCTCC


    chromosome 3 (between m433 and msu37E7)


    AthGAPAb-R CACCATGGCTTCGGTTACTT
    AthGAPAb-L TCCTGAGAATTCAGTGAAACCC


    chromosome 3 (between m457 and g2778)


    AthCDC2BG-L: GGGAAAAACGAAGTGACGTG
    AthCDC2BG-R: ATTGAACTGTGTTGGTTTCTGG


    chromosome 3 (between g2778 and m339)


    nga 707L TGAATGCGTCCAGTGAGAAG
    nga 707R CTCTCTGCCTCTCGCTGG


    chromosome 4 (between HY4 and nga1111)


    AthDET1-L: TTCAAACACCAATATCAGGCC
    AthDET1-R: GGTGAAAATGGAGGAGACGA


    chromosome 4 (between HY4 and m518)


    nga1111L GGGTTCGGTTACAATCGTGT
    nga1111R AGTTCCAGATTGAGCTTTGAGC


    chromosome 4 (between g3088 and pCITd76 )


    nga1139L TAGCCGGATGAGTTGGTACC
    nga1139R TTTTTCCTTGTGTTGCATTCC


    chromosome 4 (between HLS1 and g3713 )


    nga1107L GCGAAAAAACAAAAAAATCCA
    nga1107R CGACGAATCGACAGAATTAGG


    chromosome 4 (between HY4 and m518)


    nga1111L GGGTTCGGTTACAATCGTGT
    nga1111R AGTTCCAGATTGAGCTTTGAGC


    chromosome 5 (between g3837 and nga249)


    Ath EMC-L: AATGACGACGAGACGCTCTT
    Ath EMC-R: AACAGATCGGAAAATCGTCG

    chromosome 5(between m291and nga139)


    Ath CDPK9-L: TCAATCATTGTCCAAAACTTGG
    Ath CDPK9-R: GAAACTGACTTGGAGAAGGCA


    chromosome 5(between nga76 and Ath PhyC)


    AthS0262-R TTGCTTTTTGGTTATATTCGGA
    AthS0262-L ATCATCTGCCCATGGTTTTT


    chromosome 5 (between AthS0262 and AthS0191)


    PHYC-L: CTCAGAGAATTCCCAGAAAAATCT
    PHYC-R: AAACTCGAGAGTTTTGTCTAGATC

    chromosome 5 (between PhyC and m247)


    AthS0191-R TGATGTTGATGGAGATGGTCA
    AthS0191-L CTCCACCAATCATGCAAATG

    chromosome 5 (2 cM centromere distal to LFY3- Rob Last marker)


    MBK-5 Primers: 5' ATC ACT GTT GTT TAC CAT TA 3'
    5' GAG CAT TTC ACA GAG ACG 3'
    Product sizes:
    Col-0 207 bp
    Ler 180 bp (approx.)



    Last Updated: 12/3/96

    E-Mail Address: atgc@atgenome.bio.upenn.edu