About Us
Personnel
Outreach
Publications
Eductional Resources
PBIO-E
Contact Us

T-DNA Genotyping
Project Summary
HM Collection
Project Timeline
Target Genes
Genes in Progress
Genotyping Status
Procedure

1,001 Genomes
Project Goal
Accession
Browser
Facilities
Collaborators
MPI 1,001

cDNA
About cDNA
Project Summary
Gold Standard
Strategy
pUNI51 Vector
pHOST Vector
pIX-HALO Vector
Sequencing Status
cDNA Search
SSP Consortium
Salk 2010
FAQs

T-DNA
About T-DNA
Project Summary
Protocols
Deliverables
Project Timeline
T-DNA Express
T-DNA FAQs
Release Policy

Microarray
Overview
Protocol
Transcriptome
Genechip Search
Sample
SFP Protocol
Methylome

Educational Resources

ATGC Data
 

A New Binary pHOST Vector

We are pleased to announce the release of this plant transformation vector, pKYLX-myc9-loxP (click for Map in pdf format), to the ABRC at Ohio State University.

The vector, used in recent paper from our lab (Guo, H., and Ecker, J.R. (2003). Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor. Cell 115, 667-677.) can be recombined in vitro to rapidly create myc fusions to any of the 12000 SSP gold standard ORFs (http:/signal.salk.edu), by using Cre Recombinase.

A variety of other vectors for 2-hyb, GST- and His-tag in E. coli, as well as HA, Flag tag etc. are available from Dr. Steve Elledge Lab (selledge@genetics.med.harvard.edu) with no MTA needed. In addition, a GST-CRE plasmid is also available to make a life time supply of the Cre Recombinase enzyme in a 1 step purification method.

Please contact ABRC at Ohio State University for the pKYLX-myc9-loxp plasmid (stock number CD3-637, release date 1/26/2004).

Other pHOST vectors and the GST-CRE plasmid produced by Dr. Steve Elledge are also available from the ABRC stock center.

Reference for the original pKYLX vector: Schardl, C.L., A.D. Byrd, G. Benzion, M.A. Altschuler, D.F. Hildebrand, and A.G. Hunt. 1987. Design and construction of a versatile system for the expression of foreign genes in plants. Gene 61: 1-11.

Link to the pHOST vector map in pdf format.








© SIGnAL 2001-2013 | Home | About Us | T-DNA Express | Transcriptome | Methylome | RiceGE |
| Microarray | cDNA | T-DNA | T-DNA Genotyping | ATGC | Contact Us |