A New Binary pHOST Vector
We are pleased to announce the release of this plant transformation vector, pKYLX-myc9-loxP (click for Map in pdf format), to the ABRC at Ohio State University.
The vector, used in recent paper from our lab (Guo, H., and Ecker, J.R. (2003). Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor. Cell 115, 667-677.) can be recombined in vitro to rapidly create myc fusions to any of the 12000 SSP gold standard ORFs (http:/signal.salk.edu), by using Cre Recombinase.
A variety of other vectors for 2-hyb, GST- and His-tag in E. coli, as well as HA, Flag tag etc. are available from Dr. Steve Elledge Lab (firstname.lastname@example.org) with no MTA needed. In addition, a GST-CRE plasmid is also available to make a life time supply of the Cre Recombinase enzyme in a 1 step purification method.
Please contact ABRC at Ohio State University for the pKYLX-myc9-loxp plasmid (stock number CD3-637, release date 1/26/2004).
Other pHOST vectors and the GST-CRE plasmid produced by Dr. Steve Elledge are also available from the ABRC stock center.
Reference for the original pKYLX vector: Schardl, C.L., A.D. Byrd, G. Benzion, M.A. Altschuler, D.F. Hildebrand, and A.G. Hunt. 1987. Design and construction of a versatile system for the expression of foreign genes in plants. Gene 61: 1-11.
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