AtORFeome

The Plant Interactome project is a collaboration with Joe Ecker at the Salk Institute and Marc Vidal at Dana Farber Cancer Institute, Center for Cancer System Biology. The project aims to build a plant protein-protein interaction network by testing a matrix of 12,000 x 12,000 Arabidopsis ORFs using both yeast-2-hybrid and protein array technologies. The screen will be divided into two parts: a space I screen will assay 9000 x 9000 genes and a space II screen will be performed to incorporate the remaining 3000 genes.

Protein-protein interact ion data from space I will be available soon.

• The CCSB/Salk collaboration
• The gateway clones submitted to ABRC in pDEST-AD | in pDEST-DB.

Additional protein-protein interaction data collected from literature curation is available from the following sources:

• TAIR: http://natural.salk.edu/cgi-bin//gosearch?DB=ProteinInteraction.
• IntAct: http://www.ebi.ac.uk/intact/site/index.jsf