T-DNA Express|AtTome: Data Sources, Details and Summary ( Feb. 27, 2010 )

1. Data Sources :
    

a. Genome : AGI sequenced and assemblied pseudo-molecules, and TAIR updated and annotated v 9 (Jun-22-2009 ).

b. T-DNA/Ds FSTs :

  • 1. Salk T-DNA lines. [About Salk T-DNA] [FAQs]

    We requested that users include this reference in publications describing the use of Salk lines obtained from ABRC or NASC. Thanks!

    José M. Alonso, Anna N. Stepanova, Thomas J. Leisse, Christopher J. Kim, Huaming Chen, Paul Shinn, Denise K. Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C. Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, Michael Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzman, Laura Aguilar-Henonin, Markus Schmid, Detlef Weigel, David E. Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L. Crosby, Charles C. Berry, and Joseph R. Ecker (2003) Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana Science 301: 653-657. [Abstract] [Full Text]

  • 2. SAIL T-DNA lines. [About the SAIL lines] [Order from ABRC].
  • 3. GABI-Kat lines.
  • 4. FLAGdb/FST.
  • 5. Wisconsin T-DNA lines.
  • 6. CSHL genetrap lines.
  • 7. RIKEN T-DNA & Ds lines. [Order from RIKEN BRC]
  • 8. JIC SM lines. [About the SM lines] [NASC]
  • 9. IMA Ds lines.
  • 10. Saskatoon SK Lines.
  • 11. other FSTs from NCBI.

c. cDNAs :

  • 1. SSP (Salk, Stanford and PGEC) Consortium.

    We requested that users include this reference (below) in publications describing the use of SSP gold standard cDNA ORF clones obtained from ABRC. Thanks!

    Kayoko Yamada, Jun Lim, Joseph M. Dale, Huaming Chen, Paul Shinn, Curtis J. Palm, Audrey M. Southwick, Hank C. Wu, Christopher Kim, Michelle Nguyen, Paul Pham, Rosa Cheuk, George Karlin-Newmann, Shirley X. Liu, Bao Lam, Hitomi Sakano, Troy Wu, Guixia Yu, Molly Miranda, Hong L. Quach, Matthew Tripp, Charlie H. Chang, Jeong M. Lee, Mitsue Toriumi, Marie M. H. Chan, Carolyn C. Tang, Courtney S. Onodera, Justine M. Deng, Kenji Akiyama, Yasser Ansari, Takahiro Arakawa, Jenny Banh, Fumika Banno, Leah Bowser, Shelise Brooks, Piero Carninci, Qimin Chao, Nathan Choy, Akiko Enju, Andrew D. Goldsmith, Mani Gurjal, Nancy F. Hansen, Yoshihide Hayashizaki, Chanda Johnson-Hopson, Vickie W. Hsuan, Kei Iida, Meagan Karnes, Shehnaz Khan, Eric Koesema, Junko Ishida, Paul X. Jiang, Ted Jones, Jun Kawai, Asako Kamiya, Cristina Meyers, Maiko Nakajima, Mari Narusaka, Motoaki Seki, Tetsuya Sakurai, Masakazu Satou, Racquel Tamse, Maria Vaysberg, Erika K. Wallender, Cecilia Wong, Yuki Yamamura, Shiaulou Yuan, Kazuo Shinozaki, Ronald W. Davis, Athanasios Theologis, and Joseph R. Ecker (2003) Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome Science 302: 842-846.Abstract ] [ Full Text ] [ Supporting data ] [ Supplementary GEO Analysis Files ]

  • 2. Salk (2010 Project).
  • 3. Invitrogen/Salk Gateway Clone. [pENTR223 Seq] [pENTR223 Map]and SSP Gateway Clones [pENTR_TOPO].
  • 4. About TIGR Hypo ORFs.
  • 5. RIKEN full-length cDNAs.
  • 6. community cDNA/DNA from NCBI.
  • 7. RIKEN RAFL clone end sequences.
  • 8. Arabidopsis ESTs from NCBI.
  • 9. About GSLT cDNA.
  • 10. Peking/Yale TF clones.
  • 11. Orphan Transcripts.

d. Gene Expression Atlas :

  • 1. Salk Tome : L+: Light-grown seedlings AN: Anthers FL: Flowers-mixed stages RT: Roots SC: Suspension cell culture
  • 2. NASA Tome: GSM41324-GSM41336 from GEO, NCBI.
  • 3. Gene expression data from GEO, NCBI.

e. Others :

2. Data Details :
Salk T-DNA Columbia137,259 lines 166,127 mapped 10/28/2009
Salk T-DNA Homozygous 29,330 lines 35,240 mapped 01/26/2010
SAIL FST   62,041 mapped 10/28/2009
SAIL T-DNA Homozygous 3,494 lines 3,729 mapped 01/26/2010
Wisc FST 32,607 (22,089) 21,406 mapped 09/30/2009
FLAGdb FST Wassilevskija 41,369 37,142 mapped 06/23/2009
GABI-Kat FST Columbia 110,197 82,599 mapped (64,058 lines) 11/06/2009
GABI-Kat Confirmed 6,676 lines 9,404 mapped 11/04/2009
GABI-Kat Homozygous by Salk 756 lines 1,029 mapped 01/26/2010
JIC SM FST Landsberg 22,664 w seeds 24,206 mapped 10/28/2009
RIKEN FST N^ssen   18,622 mapped 06/23/2009
CSHL FST Columbia22,084
(16,057 lines)
21,724 mapped 10/28/2009
IMA FST Landsberg 488 lines 808 mapped 10/28/2009
Saskatoon FST Col-415,507 lines 14,941 mapped 08/17/2009
Other FST     175 mapped 06/23/2009
Total     449,635 mapped 11/09/2009

Invitrogen/Salk Gateway Clone12,042 (11,985) 06/23/2009
Salk, Stanford, PGEC (SSP) full-length cDNAs 11,732 (11,704) 06/23/2009, completed
SSP gold standard ORF clones 10,592 (10,536) 06/23/2009, completed
Salk (2010 Project) full-length cDNAs 1,278 (1,272) 06/23/2009
Salk (2010 Project) gold standard ORF clones 4,019 (3,988) 06/23/2009
RIKEN full-length cDNAs 11,497 (11,376) 07/07/2009
TIGR hypothetical genes (DNAs/cDNAs)1,054 (1,041)06/23/2009
TIGR ORFs3,095 (3,040)06/23/2009
Arabidopsis research community cDNAs (mRNA)11,352 (11,327) 01/29/2010
Arabidopsis research community Genes (DNA)1,079 (1,079)06/23/2009
Peking Yale Transcription factor reads2,057 (1,282 clones)06/23/2009
GSLT cDNA single reads 28,85906/23/2009
Orphan Transcripts63106/23/2009
ESTs on the Transcriptome1,449,016 (1,527,298)06/23/2009
Affymetrix GeneChip (GPL198) Probe Sets: 2281006/23/2009
Expts Series: 227
Expts Samples: 2987
MPSS mRNA Targets Transcriptome 80,481 (94,260) 06/25/2009
MPSS sRNA Contigs Transcriptome 148,449 06/25/2009
ASRP sRNA Transcriptome 117,14206/25/2009
JAtY TAC Transcriptome 8,29606/25/2009

3. Summary (11/09/2009) :

 SalkSalk
(HM)
Other
HM
SAILWiscGABIFLAGSKJIC SMRIKENCSHLIMACmtTotal
Mapped166127 33760 3737 61930 21406 82599 37142 14941 24206 18622 21682 805 175449635
Lines137259 28081 3368 57671 21406 64058 37142 14940 23883 18622 16057 485 175391213
Exon 15131 8879 1670 7223 3699 9359 4458 2757 3849 3652 4415 227 50 25772
Intron 7497 4100 770 3229 1697 4531 2323 1268 1056 965 1306 52 18 13316
CDSi 17903 11389 2342 9415 4997 11786 6247 3841 4492 4251 5145 271 66 27408
5' UTR 6331 3027 420 2681 945 3180 1617 1092 788 940 1096 42 12 13864
Promoter 12395 6303 349 6712 2458 8057 4243 2718 1331 1684 1834 75 25 22628
Unique
AtGenes
Identified
23955 16524 2995 14885 7579 17643 10483 6930 5832 5955 6939 371 100 30879
* Total gene number on chr1-chr5 of the TAIR genome v9 is 33,239.

4. Download Data : http://signal.salk.edu/database/transcriptome.

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