T-DNA Express|AtTome: Data Sources, Details and Summary ( May. 7, 2008 )

1. Data Sources :
    

a. Genome : AGI sequenced and assemblied pseudo-molecules, and TAIR updated and annotated v 8 (Apr-28-2008) . By comparison with Version 7, the version 8 masks 37,624 bps of vector sequences and makes 1,412 single point changes. Below is a summary of confirmation of these changes by the third party high quality SSP, Salk, TIGR, RIKEN and Community full-length/ORF sequences.


      changes
      3rd Party
      Support
      Not support
      Either
      Total
      1,412
         178
        104
           66
          8
      chr1
        267
          18
          13
             5
          0
      chr2
        156
          19
            8
           10
          1
      chr3
        237
          30
          17
           10
          3
      chr4
        544
          93
          59
           32
          2
      chr5
        208
          18
            7
             9
          2

b. T-DNA/Ds FSTs :

  • 1. Salk T-DNA lines. [About Salk T-DNA] [FAQs]

    We requested that users include this reference in publications describing the use of Salk lines obtained from ABRC or NASC. Thanks!

    José M. Alonso, Anna N. Stepanova, Thomas J. Leisse, Christopher J. Kim, Huaming Chen, Paul Shinn, Denise K. Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C. Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, Michael Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzman, Laura Aguilar-Henonin, Markus Schmid, Detlef Weigel, David E. Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L. Crosby, Charles C. Berry, and Joseph R. Ecker (2003) Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana Science 301: 653-657. [Abstract] [Full Text]

  • 2. SAIL T-DNA lines. [About the SAIL lines] [Order from ABRC].
  • 3. GABI-Kat lines.
  • 4. FLAGdb/FST.
  • 5. Wisconsin T-DNA lines.
  • 6. CSHL genetrap lines.
  • 7. RIKEN T-DNA & Ds lines. [Order from RIKEN BRC]
  • 8. JIC SM lines. [About the SM lines] [NASC]
  • 9. IMA Ds lines.
  • 10. other FSTs from NCBI.

c. cDNAs :

  • 1. SSP (Salk, Stanford and PGEC) Consortium.

    We requested that users include this reference (below) in publications describing the use of SSP gold standard cDNA ORF clones obtained from ABRC. Thanks!

    Kayoko Yamada, Jun Lim, Joseph M. Dale, Huaming Chen, Paul Shinn, Curtis J. Palm, Audrey M. Southwick, Hank C. Wu, Christopher Kim, Michelle Nguyen, Paul Pham, Rosa Cheuk, George Karlin-Newmann, Shirley X. Liu, Bao Lam, Hitomi Sakano, Troy Wu, Guixia Yu, Molly Miranda, Hong L. Quach, Matthew Tripp, Charlie H. Chang, Jeong M. Lee, Mitsue Toriumi, Marie M. H. Chan, Carolyn C. Tang, Courtney S. Onodera, Justine M. Deng, Kenji Akiyama, Yasser Ansari, Takahiro Arakawa, Jenny Banh, Fumika Banno, Leah Bowser, Shelise Brooks, Piero Carninci, Qimin Chao, Nathan Choy, Akiko Enju, Andrew D. Goldsmith, Mani Gurjal, Nancy F. Hansen, Yoshihide Hayashizaki, Chanda Johnson-Hopson, Vickie W. Hsuan, Kei Iida, Meagan Karnes, Shehnaz Khan, Eric Koesema, Junko Ishida, Paul X. Jiang, Ted Jones, Jun Kawai, Asako Kamiya, Cristina Meyers, Maiko Nakajima, Mari Narusaka, Motoaki Seki, Tetsuya Sakurai, Masakazu Satou, Racquel Tamse, Maria Vaysberg, Erika K. Wallender, Cecilia Wong, Yuki Yamamura, Shiaulou Yuan, Kazuo Shinozaki, Ronald W. Davis, Athanasios Theologis, and Joseph R. Ecker (2003) Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome Science 302: 842-846.Abstract ] [ Full Text ] [ Supporting data ] [ Supplementary GEO Analysis Files ]

  • 2. Salk (2010 Project).
  • 3. Invitrogen/Salk Gateway Clone. [pENTR223 Seq] [pENTR223 Map]and SSP Gateway Clones [pENTR_TOPO].
  • 4. About TIGR Hypo ORFs.
  • 5. RIKEN full-length cDNAs.
  • 6. community cDNA/DNA from NCBI.
  • 7. RIKEN RAFL clone end sequences.
  • 8. Arabidopsis ESTs from NCBI.
  • 9. About GSLT cDNA.
  • 10. Peking/Yale TF clones.
  • 11. Orphan Transcripts.

d. Gene Expression Atlas :

  • 1. Salk Tome : L+: Light-grown seedlings AN: Anthers FL: Flowers-mixed stages RT: Roots SC: Suspension cell culture
  • 2. NASA Tome: GSM41324-GSM41336 from GEO, NCBI.
  • 3. Gene expression data from GEO, NCBI.

e. Others :

2. Data Details :
Salk T-DNA Columbia  151,534 mapped 07/09/2007
Salk T-DNA Homozygous 19,851 lines 22,260 mapped 05/01/2008
Other T-DNA Homozygous
(SAIL) (Wisc)
25 lines 27mapped 03/12/2007
SAIL FST   57,242 mapped 03/12/2007
Wisc FST   10,470 mapped 07/09/2007
FLAGdb FST Wassilevskija 36,287 31,744 mapped 07/09/2007
GABI-Kat FST Columbia 110,197 63,887 mapped 07/09/2007
GABI-Kat Confirmed   5,446 mapped 10/08/2007
JIC SM FST Landsberg 22,664 w seeds 23,573 mapped 07/09/2007
RIKEN FST N^ssen   18,566 mapped 07/09/2007
CSHL FST Columbia22,084
(16,018 lines)
21,661 mapped 07/09/2007
IMA FST Landsberg 488 lines 822 mapped 07/09/2007
Other FST     175 mapped 07/09/2007
Total     379,674 mapped 07/09/2007

Invitrogen/Salk Gateway Clone12,143 (12,090) 02/08/2008
Salk, Stanford, PGEC (SSP) full-length cDNAs 11,727 (11,704) 03/12/2007, completed
SSP gold standard ORF clones 10,586 (10,537) 03/12/2007, completed
Salk (2010 Project) full-length cDNAs 1,280 (1,272) 03/12/2007
Salk (2010 Project) gold standard ORF clones 3,774 (3,749) 04/15/2008
RIKEN full-length cDNAs 9,934 (9,864) 03/12/2007
TIGR hypothetical genes (DNAs/cDNAs)1,055 (1,041)04/04/2008
TIGR ORFs3,070 (3,041)04/04/2008
Arabidopsis research community cDNAs (mRNA)11,176 (11,0386 05/07/2008
Arabidopsis research community Genes (DNA)1,080 (1,080)03/12/2007
Peking Yale Transcription factor reads2,057 (1,282 clones)03/12/2007
RIKEN cDNA end sequences19,60603/12/2007
GSLT cDNA single reads 28,81603/12/2007
Orphan Transcripts59103/12/2007
ESTs on the Transcriptome713,598 (730,932)03/12/2007
Affymetrix GeneChip (GPL198) Probe Sets: 2281008/30/2007
Expts Series: 227
Expts Samples: 2987
MPSS mRNA Targets Transcriptome 80,486 (94,265) 03/12/2007
MPSS sRNA Contigs Transcriptome 148,456 03/12/2007
ASRP sRNA Transcriptome 262,74203/12/2007
JAtY TAC Transcriptome 9,31403/12/2007

3. Summary (05/01/2008) :

 SalkSalk
(HM)
SAILWiscGABIFLAGJIC SMRIKENCSHLIMACmtsTotal
Mapped150355 22737 57242 10459 63887 31744 23573 18566 21661 822 175378484
Exon 14864 7081 7680 2011 8535 3885 3701 3593 4369 224 45 24689
Intron 7531 3098 3481 966 4188 2030 1052 985 1303 52 21 12765
CDSi 17668 9264 9964 2843 11038 5507 4357 4201 5106 268 64 26567
5' UTR 6132 2012 2860 562 2775 1408 746 945 1102 44 11 12663
Promoter 12038 4361 6515 1456 7046 3744 1317 1684 1812 83 23 21157
Unique
AtGenes
Identified
23558 13493 15236 4569 16534 9373 5662 5902 6886 376 95 30280
* Total gene number on chr1-chr5 of the AGI/TAIR genome v8 is 33,003.

4. Download Data : http://signal.salk.edu/database/transcriptome.

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